Loss of DCC gene expression during ovarian tumorigenesis: relation to tumour differentiation and progression

To clarify the possible role of DCC gene alteration in ovarian neoplasias, we immunohistochemically investigated 124 carcinomas, as well as 55 cystadenomas and 41 low malignant potential (LMP) tumours and compared the results with those for p53 protein expression, clinicopathological factors and survival. A combination of the reverse transcription polymerase chain reaction (RT-PCR) and Southern blot hybridization (SBH) for DCC mRNA levels was also carried out on 26 malignant, five LMP, eight benign and seven normal ovarian samples. Significantly decreased levels of overall DCC values in carcinomas compared with benign and LMP lesions were revealed by both immunohistochemical and RT-PCR/SBH assays. Similar findings were also noted when subdivision was into serous and mucinous categories. In carcinomas, reduction or loss of DCC expression was significantly related to the serous phenotype (serous vs non-serous, P< 0.0001), a high histological grade (grade 1 vs 2 or 3, P< 0.02) and a more advanced stage (FIGO stage I vs II/III/IV, P = 0.0083), while no association was noted with survival. Although p53 immunopositivity demonstrated significant stepwise increase from benign through to malignant lesions, there was no clear association with DCC score values. The results indicated that impaired DCC expression may play an important role in ovarian tumorigenesis. In ovarian carcinomas, the altered expression is closely linked with tumour differentiation and progression. © 2000 Cancer Research Campaig

β-Catenin mutations and aberrant nuclear expression during endometrial tumorigenesis

To clarify the possible role of aberrant β-catenin expression during endometrial tumorigenesis, a total of 199 cases of endometrial carcinomas (endometrioid type), as well as 37 cases of simple/complex and 32 of atypical hyperplasias, was consecutively investigated for immunohistochemistry, along with 141 normal endometrial samples distant from carcinomas. Of 199 carcinoma cases, 73 tumours as well as 44 normal samples were also analysed using a combination of RT-PCR and Southern blot hybridization, Western blot, and mutation gene assays. Cell membrane β-catenin immunoreactivity showed a stepwise decrease from normal, through atypical hyperplasia, to grade 3 carcinomas. In contrast, the nuclear accumulation in atypical hyperplasias and grade 1 or 2 tumours was higher than in simple/complex hyperplasias. Mutations in exon 3 of the β-catenin gene involving codons 33, 34, 37, 41, and 45 were observed in 16 (22.9%) of 70 endometrial carcinomas, as well as 3 (12.5%) of 24 atypical hyperplasias, the results being significantly related to low membrane and high nuclear immunoreactivity but not relative mRNA expression levels, suggesting that the gene mutations may be closely associated with changes in subcellular distribution. In addition to significant association between β-catenin mutation and low grade histological malignancy (P = 0.048), the mutations were detected in none of 15 and 13 (26%) of 50 tumours with or without lymph node metastasis, the difference being significant (P = 0.027). These findings suggest that β-catenin abnormalities may play an important role in a relatively early event during the endometrial hyperplasia-carcinoma sequence. © 2001 Cancer Research Campaign http://www.bjcancer.co

Sub-Arcsecond Imaging of 3C123:108-GHz Continuum Observations of the Radio Hotspots

We present the results of sub-arcsecond 108 GHz continuum interferometric observations toward the radio luminous galaxy 3C123. Using multi-array observations, we utilize the high u,v dynamic range of the BIMA millimeter array to sample fully spatial scales ranging from 0.5" to 50". This allows us to make one-to-one comparisons of millimeter-wavelength emission in the radio lobes and hotspots to VLA centimeter observations at 1.4, 4.9, 8.4, and 15 GHz. At 108 GHz, the bright, eastern double hotspot in the southern lobe is resolved. This is only the second time that a multiple hotspot region has been resolved in the millimeter regime. We model the synchrotron spectra of the hotspots and radio lobes using simple broken power-law models with high energy cutoffs, and discuss the hotspot spectra and their implications for models of multiple hotspot formation.Comment: 16 pages, 3 Figures, ApJ Accepte

Asymmetric Field Profile in Bose Glass Phase of Irradiated YBa2Cu3O7-d: Loss of Interlayer Coherence around 1/3 of Matching Field

Magneto-optical imaging in YBa2Cu3O7-d with tilted columnar defects (CD's) shows an asymmetric critical-state field profile. The observed hysteretic shift of the profile ridge (trough) from the center of the sample is explained by in-plane magnetization originated from vortex alignment along CD's. The extracted ratio of the in-plane to out-of-plane magnetization component has a maximum at 1/5 of matching field ($B_\Phi$) and disappears above $B_\Phi/3$, suggesting a reduction of interlayer coherence well bellow $B_\Phi$ in the Bose glass phase. Implications are discussed in comparison with the vortex liquid recoupling observed in irradiated Bi2Sr2CaCu2O8+y.Comment: Revtex, 4 pages, 5 figures, also see a movie at (http://www.ap6.t.u-tokyo.ac.jp/kitaka/Research/d-line/index_e.htm). This manuscript will appear in Phys. Rev. Let

Exclusive Measurement of the Nonmesonic Weak Decay of ^{5}_{\Lambda}He Hypernucleus

We performed a coincidence measurement of two nucleons emitted from the nonmesonic weak decay (NMWD) of ^{5}_{\Lambda}He formed via the ^{6}Li(\pi^+,K^+) reaction. The energies of two nucleons and the pair number distributions in the opening angle between them were measured. In both np and nn pairs, we observed a clean back-to-back correlation coming from the two-body decay of \Lambda p --> n p and \Lambda n --> n n, respectively. The ratio of the nucleon pair numbers was N_{nn}/N_{np}=0.45 \pm 0.11(stat)\pm 0.03(syst) in the kinematic region of cos(theta_{NN}) < -0.8. Since each decay mode was exclusively detected, the measured ratio should be close to the ratio of \Gamma(\Lambda p --> np)/\Gamma(\Lambda n --> nn). The ratio is consistent with recent theoretical calculations based on the heavy meson/direct quark exchange picture.Comment: Submitted to Phys. Rev. lett., 4 pages, 3 figure

Application of Pulsed Field Gel Electrophoresis to Determine γ-ray-induced Double-strand Breaks in Yeast Chromosomal Molecules

The frequency of DNA double-strand breaks (dsb) was determined in yeast cells exposed to γ-rays under anoxic conditions. Genomic DNA of treated cells was separated by pulsed field gel electrophoresis, and two different approaches for the evaluation of the gels were employed: (1) The DNA mass distribution profile obtained by electrophoresis was compared to computed profiles, and the number of DSB per unit length was then derived in terms of a fitting procedure; (2) hybridization of selected chromosomes was performed, and a comparison of the hybridization signals in treated and untreated samples was then used to derive the frequency of dsb